Find Your Sweet Spot in One Experiment, Not Ten.
Stop guessing your way through One-Factor-at-a-Time (OFAT) trials. BioDOE's 3-Panel Design Studio runs real ANOVA and response-surface optimization under the hood for assays, bioprocessing, and formulations — no statistics background needed to run it, none skipped to get there.
Built for Biological Complexity
From high-throughput screening and formulation buffers to equipment constraints in bioprocessing suites.
1. Biological Factor Setup
Specify 2-level continuous ranges (pH, Substrate Concentration) and discrete multi-option choices (Media Types, Enzyme Strains, Buffer Components).
Explore Factor Setup2. Hard-to-Change Factors
Flag settings that are slow or expensive to change — like incubation temperature or which bioreactor vessel you're using — so the design groups runs together instead of resetting equipment between every single run.
Explore Hard-to-Change Support3. Balance Competing Goals
Automatically finds the best settings when your goals pull in different directions — for example, maximizing yield while minimizing impurity — instead of you weighing the tradeoffs by hand.
Explore 3D Surface MeshAccelerating Discovery Across Every Assay & Bioprocess
Whether optimizing CHO cell culture, Immunoassay Development (ELISA & Potency Assays), or formulation stability, BioDOE reduces experimental runs by up to 75%.
CHO Cell Culture Media Optimization
Simultaneously evaluate Glucose, Amino Acid Feeds, and Temperature Shift timing to boost monoclonal antibody titers while maintaining 95%+ cell viability.
Bioprocess Case StudyImmunoassay & Bioassay Development
Optimize ELISA, HTRF, and Cell-Based Potency Assays. Simultaneously screen antibody coating levels, detection conjugate dilutions, blocking agents, and incubation times to maximize signal-to-noise (S/N) ratio and dynamic range.
Immunoassay OptimizationRecombinant Protein & Enzyme Expression
Screen induction timing, IPTG/Methanol concentration, and Dissolved Oxygen levels. Account for hard-to-change incubator temps via Split-Plot designs.
Expression WorkflowAssay Buffer & Formulation Stability
Formulate high-stability biologics. Optimize pH, ionic strength, surfactants, and cryoprotectants to prevent aggregation through freeze-thaw cycles.
Formulation MatrixGene Therapy & Viral Vector Transfection
Optimize AAV or Lentivirus production by balancing plasmid ratios, transfection reagent concentrations, and harvest timing for maximum viral titer.
Gene Therapy SolutionsA well-designed screening experiment tests every factor together in a handful of runs — surfacing interactions between factors, like how Glucose and Temperature affect each other, that a traditional one-factor-at-a-time approach can take months to stumble onto, if it ever does.
Simple Plans Built for Every Stage of Research
Start free, upgrade as your experimental volume grows. No expensive desktop licenses required.
- 3 Designs + 3 Analyses / Month
- Screening & Mapping Designs (Find What Matters / Map the Shape)
- Full Statistical Analysis (ANOVA, Pareto, Actual vs. Predicted)
- CSV Import/Export & PNG/SVG Figure Export
- Secure Sign-In
- Unlimited Designs & Analyses
- Optimization Designs (Find the Peak)
- Hard-to-Change Factor Support
- Multi-Goal Optimization (Optimal Settings Recommendation)
- 3D Surface & Contour Plots
Ready to Transform Your Biological Optimization?
Join leading life scientists, bioprocess engineers, and biotech researchers using BioDOE to design, execute, and optimize multi-factor experiments in minutes.